Video Codec : MPEG4
Video Bitrate : 448 Kbps
Audio Codec : AAC
Audio Bitrate : 64 Kbps
Picking up shortly after the end of Hostel, the film opens with Paxton (Jay Hernandez) in seclusion with his girlfriend Stephanie (Jordan Ladd). The two argue, and the next morning Stephanie wakes up to discover Paxton's decapitated body seated at the kitchen table, with their cat licking the blood from his neck. Meanwhile, in Slovakia, a mysterious package is delivered to Sasha (Milan K?ažko), the owner of Elite Hunting. From the reaction of Sasha's hounddogs and the size of the box, it is left to the audience to deduce that this package contains Paxton's head (which was missing from the scene of the decapitation). In Italy, art students Beth (Lauren German), Whitney (Bijou Phillips) and Lorna (Heather Matarazzo) are convinced by Axelle (Vera Jordanova), a nude model they are sketching, to join her on a vacation to a luxurious spa. The four travel to a small Slovakian village and check into the local hostel, where the desk clerk uploads their passport photos to an auction website, where hotshot American businessman Todd (Richard Burgi) bids on Whitney and Beth for himself and his passive best friend Stuart (Roger Bart).
Later that night, at the village's "Harvest Festival", Lorna discovers that Beth has inherited a vast fortune from her mother. Stuart approaches Beth and the two share a friendly, if awkward, conversation. An intoxicated Lorna leaves to go on a boat ride with Roman, a charismatic local, who proceeds to kidnap Lorna with the help of two accomplices. A local walks up to Beth and asks her for a dance, she declines. He responds with "I could have helped you." Beth doesn't understand and shortly afterwards the Hostel clerk approaches and tells her that "He won't bother you anymore." Beth and Whitney leave the party, while Axelle volunteers to stay behind and wait for Lorna.
The next morning the three girls head to the local spa to relax. Basking in the relaxing atmosphere of the hot springs, Beth is able to fall somewhat at ease and dozes off. Meanwhile, a naked Lorna is shackled upside-down in a large room, where a woman (Monika Malacova) named Mrs. Bathory enters, undresses and lies beneath Lorna, then proceeds to slowly kill Lorna, cutting her with a long scythe and bathing in her blood, then slitting her throat with a sickle.