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Name:Computational Methods for Mass Spectrometry Proteomics~tqw~ darksiderg

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Computational Methods for Mass Spectrometry Proteomics

General Information
Type.................: Ebook
Part Size............: 4,653,208 bytes

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Posted by............: ~tqw~

Release Notes
Proteomics is the study of the subsets of proteins present in different parts of
an organism and how they change with time and varying conditions. Mass
spectrometry is the leading technology used in proteomics, and the field relies
heavily on bioinformatics to process and analyze the acquired data. Since
recent years have seen tremendous developments in instrumentation and
proteomics-related bioinformatics, there is clearly a need for a solid
introduction to the crossroads where proteomics and bioinformatics meet.

Computational Methods for Mass Spectrometry Proteomics describes the different
instruments and methodologies used in proteomics in a unified manner. The
authors put an emphasis on the computational methods for the different phases of
a proteomics analysis, but the underlying principles in protein chemistry and
instrument technology are also described. The book is illustrated by a number of
figures and examples, and contains exercises for the reader. Written in an
accessible yet rigorous style, it is a valuable reference for both
informaticians and biologists.

Computational Methods for Mass Spectrometry Proteomics is suited for advanced
undergraduate and graduate students of bioinformatics and molecular biology with
an interest in proteomics. It also provides a good introduction and reference
source for researchers new to proteomics, and for people who come into more
peripheral contact with the field.

Table Of Contents

Preface ix
Acknowledgements xi
1 Protein, proteome, and proteomics 1
2 Protein separation – 2D gel electrophoresis 31
3 Protein digestion 43
4 Peptide separation – HPLC 53
5 Fundamentals of mass spectrometry 67
6 Mass spectrometry – MALDI-TOF 81
7 Protein identification and characterization by MS 97
8 Tandem MS or MS/MS analysis 119
9 Fragmentation models 141
10 Identification and characterization by MS/MS 153
11 Spectral comparisons 159
12 Sequential comparison – de novo sequencing 179
13 Database searching for de novo sequences 193
14 Large-scale proteomics 211
15 Quantitative MS-based proteomics 229
16 Peptides to proteins 243
17 Top-down proteomics 249
18 Standards 255
Bibliography 265
Index 277

Product Details

* ISBN: 0470512970
* ISBN-13: 9780470512975
* Format: Hardcover, 296pp
* Publisher: Wiley, John & Sons, Incorporated
* Pub. Date: February 2008

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